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ROS Live Cell Imaging tijdens neuronale ontwikkeling

Article DOI: 10.3791/62165-v • 09:25 min • February 9th, 2021
February 9th, 2021 •
1,2, 1,2, 1,2,3
1Department of Biological Sciences, Purdue University, 2Purdue Institute for Integrative Neuroscience, Purdue University, 3Bindley Bioscience Center, Purdue University

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Dit protocol beschrijft het gebruik van een genetisch gecodeerd waterstofperoxide (H2O2)-biosensor in gekweekte zebravisneuronen en larven voor het beoordelen van de fysiologische signaleringsrollen van H2O2 tijdens de ontwikkeling van het zenuwstelsel. Het kan worden toegepast op verschillende celtypen en worden aangepast met experimentele behandelingen om reactieve zuurstofsoorten (ROS) in de algemene ontwikkeling te bestuderen.

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Neurowetenschappen ROS waterstofperoxide roGFP2-Orp1 biosensor RGC zebravis neuronale ontwikkeling
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