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Kemisk dimerisering-induceret protein kondensater på telomerer
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Kemisk dimerisering-induceret protein kondensater på telomerer

Article DOI: 10.3791/62173-v • 08:52 min • April 12th, 2021
April 12th, 2021 •
1, 2, 1
1Department of Biological Sciences, Mellon College of Science, Carnegie Mellon University, 2Department of Chemistry, School of Arts and Sciences, University of Pennsylvania

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Denne protokol illustrerer et kemisk induceret protein dimerization system til at skabe kondensater på kromatin.  Dannelsen af promyelocytic leukæmi (PML) nukleare organ på telomerer med kemisk dimerizers er demonstreret. Dråbevækst, opløsning, lokalisering og sammensætning overvåges med levende cellebilleddannelse, immunofluorescence (IF) og fluorescens in situ hybridisering (FISH).

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Biologi Udgave 170 kondensater væske-væske fase adskillelse kemisk dimerizer lokal kondens telomerer PML nukleare organ
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