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Chemische dimerisatie-geïnduceerde eiwitcondensaten op telomeren
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Chemische dimerisatie-geïnduceerde eiwitcondensaten op telomeren

Article DOI: 10.3791/62173-v • 08:52 min • April 12th, 2021
April 12th, 2021 •
1, 2, 1
1Department of Biological Sciences, Mellon College of Science, Carnegie Mellon University, 2Department of Chemistry, School of Arts and Sciences, University of Pennsylvania

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Dit protocol illustreert een chemisch geïnduceerd eiwitdimeerisatiesysteem om condensaten op chromatine te maken.  De vorming van promyelocytaire leukemie (PML) nucleair lichaam op telomeren met chemische dimerizers is aangetoond. Druppelgroei, oplossing, lokalisatie en samenstelling worden gemonitord met live cell imaging, immunofluorescentie (IF) en fluorescentie in situ hybridisatie (FISH).

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Biologie Nummer 170 condensaten vloeistof-vloeistof fasescheiding chemische dimerizer lokale condensatie telomeren PML nucleair lichaam
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