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Visualisering og kvantificering af endonuklease-baseret stedsspecifik DNA-skade
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Visualisering og kvantificering af endonuklease-baseret stedsspecifik DNA-skade

Article DOI: 10.3791/62175-v • 10:59 min • August 21st, 2021
August 21st, 2021 •
*1, *1, 1, 1
1Institute of Pathology, Faculty of Medicine, University of Szeged
* These authors contributed equally

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Denne artikel introducerer væsentlige trin i immunostaining og chromatin immunoprecipitation. Disse protokoller bruges ofte til at studere DNA-skaderelaterede cellulære processer og til at visualisere og kvantificere rekrutteringen af proteiner, der er impliceret i DNA-reparation.

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Biologi Problem 174 DNA-reparation chromatin immunoprecipitation fluorescerende mikroskopi stedsspecifikke DSB'er immunostaining
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