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Visualiseren en kwantificeren van endonuclease-gebaseerde site-specifieke DNA-schade
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Visualiseren en kwantificeren van endonuclease-gebaseerde site-specifieke DNA-schade

Article DOI: 10.3791/62175-v • 10:59 min • August 21st, 2021
August 21st, 2021 •
*1, *1, 1, 1
1Institute of Pathology, Faculty of Medicine, University of Szeged
* These authors contributed equally

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Dit artikel introduceert essentiële stappen van immunostaining en chromatine immunoprecipitatie. Deze protocollen worden vaak gebruikt om DNA-schadegerelateerde cellulaire processen te bestuderen en om de rekrutering van eiwitten die betrokken zijn bij DNA-reparatie te visualiseren en te kwantificeren.

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Biologie DNA reparatie chromatine immunoprecipitatie fluorescerende microscopie site-specifieke DSB's immunostaining
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