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Journal / Biochemistry / Misurazioni basate sulla fluorescenza dello…
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Biochemistry

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JoVE Journal Biochemistry
Misurazioni basate sulla fluorescenza dello scambio di fosfatidilserina / fosfatidilinositolo 4-fosfato tra le membrane
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Misurazioni basate sulla fluorescenza dello scambio di fosfatidilserina / fosfatidilinositolo 4-fosfato tra le membrane

Article DOI: 10.3791/62177-v • 08:49 min • March 14th, 2021
March 14th, 2021 •
*1, *1, 1, 1
1Université Côte d’Azur, Centre National de la Recherche Scientifique, Institut de Pharmacologie Moléculaire et Cellulaire
* These authors contributed equally

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Qui, descriviamo protocolli che utilizzano sensori lipidici fluorescenti e liposomi per determinare se una proteina estrae e trasporta fosfatidilserina o fosfatidilinositolo 4-fosfato in vitro.

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Biochimica Numero 169 proteina di trasferimento lipidico proteina correlata all'OSBP fosfatidilserina fosfatidilinositolo 4-fosfato fluorescenza proteina ricombinante liposoma
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