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JoVE Journal Developmental Biology
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Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity

Article DOI: 10.3791/62182-v • 10:03 min • January 30th, 2021
January 30th, 2021 •
1,2, 3, 4, 5, 6, 1
1Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, 2Department of Dermatology, Brigham and Women's Hospital, Harvard Medical School, 3Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine Baltimore, 4Department of Pathology, Albert Einstein College of Medicine, 5Department of Pediatrics, Vanderbilt University Medical Center, 6Department of Drug Discovery and Biomedical Sciences, College of Pharmacy, University of South Carolina

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Here we describe a protocol for the systematic cultivation of epidermal spheroids in 3D suspension culture. This protocol has wide-ranging applications for use in a variety of epithelial tissue types and for the modeling of several human diseases and conditions.

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High Throughput Epidermal Spheroid Culture System Keratinocyte Stem Cells Plasticity Cultivation Maintenance Spheroid Replating Assay Wash Medium Neonatal Foreskin Wash Media Sterile Petri Dish Adipose Tissue Connective Tissues Dermal Layer Dispase Enzyme Incubator Dermis Layer 0.25% Trypsin-EDTA Soybean Trypsin Inhibitor Cell Suspension Complete KSFM-SCM
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