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JoVE Journal Developmental Biology
Creazione di un sistema di coltura sferoide epidermica ad alto rendimento per modellare la plasticità delle cellule staminali dei cheratinociti
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Creazione di un sistema di coltura sferoide epidermica ad alto rendimento per modellare la plasticità delle cellule staminali dei cheratinociti

Article DOI: 10.3791/62182-v • 10:03 min • January 30th, 2021
January 30th, 2021 •
1,2, 3, 4, 5, 6, 1
1Department of Pathology, Microbiology and Immunology, University of South Carolina School of Medicine, 2Department of Dermatology, Brigham and Women's Hospital, Harvard Medical School, 3Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine Baltimore, 4Department of Pathology, Albert Einstein College of Medicine, 5Department of Pediatrics, Vanderbilt University Medical Center, 6Department of Drug Discovery and Biomedical Sciences, College of Pharmacy, University of South Carolina

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Qui descriviamo un protocollo per la coltivazione sistematica di sferoidi epidermici in coltura di sospensione 3D. Questo protocollo ha applicazioni ad ampio raggio per l'uso in una varietà di tipi di tessuto epiteliale e per la modellazione di diverse malattie e condizioni umane.

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Biologia dello sviluppo Numero 167 Anoikis plasticità cellulare epitelio cellule staminali epidermiche epidermosfera cheratinociti umani sferoidi coltura 3D guarigione delle ferite
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