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Journal / Immunology and Infection / Surveillance de l’activité de l’élastase…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Surveillance de l’activité de l’élastase neutrophile et de la cathepsine G dans les échantillons d’expectorations humaines
High and Low Throughput Screens with Root-knot Nematodes Meloidogyne spp.
Journal (Immunology and Infection)
High and Low Throughput Screens with Root-knot Nematodes Meloidogyne spp.
Isolation of Brain and Spinal Cord Mononuclear Cells Using Percoll Gradients
Journal (Immunology and Infection)
Isolation of Brain and Spinal Cord Mononuclear Cells Using Percoll Gradients
 
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Surveillance de l’activité de l’élastase neutrophile et de la cathepsine G dans les échantillons d’expectorations humaines

Article DOI: 10.3791/62193-v • 09:23 min • May 21st, 2021
May 21st, 2021 •
*1,2, *1,2,3,4, 1,2,5,6,7, 1,3,8
1Translational Lung Research Center Heidelberg (TLRC), German Center for Lung Research (DZL), 2Dept. of Translational Pulmonology, University of Heidelberg, 3Molecular Medicine Partnership Unit (MMPU), European Molecular Biology Laboratory (EMBL), University of Heidelberg, 4Faculty of Biosciences, Collaboration for Joint Ph.D. Degree between EMBL and Heidelberg University, University of Heidelberg, 5Dept. of Pediatric Pulmonology, Immunology and Critical Care Medicine, Charité – Universitätsmedizin Berlin, 6Berlin Institute of Health (BIH), 7German Center for Lung Research (DZL), Associated Partner Site, Berlin, 8Dept. of Chemical Physiology and Biochemistry, Oregon Health and Science University
* These authors contributed equally

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Summary
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Les protocoles décrits ci-dessus fournissent un guide pour visualiser et quantifier l’activité des protéases neutrophiles dans les expectorations humaines. Les applications de cette analyse vont de l’évaluation des traitements anti-inflammatoires à la validation des biomarqueurs, au dépistage des médicaments et aux études cliniques de grandes cohortes.

Tags

Immunologie et infection Numéro 171 Fibrose kystique maladie pulmonaire inflammation protéases rapporteurs FRET élastase neutrophile cathepsine G diagnostics
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