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Journal / Immunology and Infection / Monitoramento de Elastase e Cathepsin G Atividade …
JoVE Journal
Immunology and Infection

This content is Open Access.

JoVE Journal Immunology and Infection
Monitoramento de Elastase e Cathepsin G Atividade em Amostras de Espiuma Humana
Visualization and Quantification of High-Dimensional Cytometry Data using Cytofast and the Upstream Clustering Methods FlowSOM and Cytosplore
Journal (Immunology and Infection)
Visualization and Quantification of High-Dimensional Cytometry Data using Cytofast and the Upstream Clustering Methods FlowSOM and Cytosplore
Protocol for Production of a Genetic Cross of the Rodent Malaria Parasites
Journal (Immunology and Infection)
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Monitoramento de Elastase e Cathepsin G Atividade em Amostras de Espiuma Humana

Article DOI: 10.3791/62193-v • 09:23 min • May 21st, 2021
May 21st, 2021 •
*1,2, *1,2,3,4, 1,2,5,6,7, 1,3,8
1Translational Lung Research Center Heidelberg (TLRC), German Center for Lung Research (DZL), 2Dept. of Translational Pulmonology, University of Heidelberg, 3Molecular Medicine Partnership Unit (MMPU), European Molecular Biology Laboratory (EMBL), University of Heidelberg, 4Faculty of Biosciences, Collaboration for Joint Ph.D. Degree between EMBL and Heidelberg University, University of Heidelberg, 5Dept. of Pediatric Pulmonology, Immunology and Critical Care Medicine, Charité – Universitätsmedizin Berlin, 6Berlin Institute of Health (BIH), 7German Center for Lung Research (DZL), Associated Partner Site, Berlin, 8Dept. of Chemical Physiology and Biochemistry, Oregon Health and Science University
* These authors contributed equally

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Os protocolos aqui descritos fornecem um guia para visualizar e quantificar a atividade de proteases neutrófilos na escarro humana. As aplicações dessa análise abrangem desde a avaliação de tratamentos anti-inflamatórios, até validação de biomarcadores, triagem de medicamentos e grandes estudos clínicos de coorte.

Tags

Imunologia e Infecção Problema 171 Fibrose Cística Doença Pulmonar Inflamação proteases repórteres FRET elastase neutrófilo cathepsina G diagnósticos
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