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Journal / Biochemistry / Быстрая подготовка сетки для…
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Biochemistry
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JoVE Journal Biochemistry
Быстрая подготовка сетки для криоэлектроно-микроскопии с временным разрешением
OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
Journal (Biochemistry)
OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
Journal (Biochemistry)
PCR Mutagenesis, Cloning, Expression, Fast Protein Purification Protocols and Crystallization of the Wild Type and Mutant Forms of Tryptophan Synthase
 
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Быстрая подготовка сетки для криоэлектроно-микроскопии с временным разрешением

Article DOI: 10.3791/62199-v • 10:05 min • November 6th, 2021
November 6th, 2021 •
1, 2, 3, 1
1School of Biomedical Sciences, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology, University of Leeds, 2School of Molecular and Cellular Biology, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology, University of Leeds, 3Department of Physiological Sciences, Eastern Virginia Medical School

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Здесь мы предоставляем подробный протокол для использования устройства быстрого создания сетки как для быстрого изготовления сетки, так и для быстрого смешивания и замораживания для проведения экспериментов с временным разрешением.

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Биохимия выпуск 177 крио-ЭМ пробоподготовка с временным разрешением
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