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JoVE Journal Developmental Biology

Генерация и расширение кардиомиоцитов человека из мононуклеарных клеток периферической крови пациента

FACS Enrichment of Zebrafish Gut Cell Suspension for scRNAseq

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Detection and Quantitation of Label-Retaining Cells in Mouse Incisors using a 3D Reconstruction Approach after Tissue Clearing

Journal (Developmental Biology)

Detection and Quantitation of Label-Retaining Cells in Mouse Incisors using a 3D Reconstruction Approach after Tissue Clearing

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Генерация и расширение кардиомиоцитов человека из мононуклеарных клеток периферической крови пациента

Article DOI: 10.3791/62206-v • 05:38 min • February 12th, 2021

February 12th, 2021 •

Shiqiao Ye^1,2, Xiaoping Wan³, Juan Su^1,2, Akshar Patel^1,2,4, Blake Justis^1,2,5, Isabelle Deschênes³, Ming-Tao Zhao^1,2,5,6

¹Center for Cardiovascular Research, The Abigail Wexner Research Institute, Nationwide Children’s Hospital, ²The Heart Center, Nationwide Children’s Hospital, ³Department of Physiology and Cell Biology, The Ohio State University College of Medicine, ⁴Department of Anatomy, The Ohio State University College of Medicine, ⁵MCDB Graduate Program, The Ohio State University, ⁶Department of Pediatrics, The Ohio State University College of Medicine

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Здесь мы представляем протокол для надежной генерации и расширения кардиомиоцитов человека из мононуклеарных клеток периферической крови пациента.

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Биология развития выпуск 168 мононуклеарные клетки периферической крови PBMCs индуцированные человеком плюрипотентные стволовые клетки iPSCs сердечная дифференцировка перепрограммирование iPSC расширение кардиомиоцитов.

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