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Neuroscience

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JoVE Journal Neuroscience
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In vitro Quantitative Imaging Assay for Phagocytosis of Dead Neuroblastoma Cells by iPSC-Macrophages

Article DOI: 10.3791/62217-v • 09:48 min • February 14th, 2021
February 14th, 2021 •
1,2,3, 2, 1, 2, 1
1James Martin Stem Cell Facility, Sir William Dunn School of Pathology, University of Oxford, 2Alzheimer’s Research UK Oxford Drug Discovery Institute, Nuffield Department of Medicine Research Building, University of Oxford, 3UK Dementia Research Institute, Cardiff University

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Summary
Automatic Translation

Neurodegenerative diseases are associated with dysregulated microglia functions. This article outlines an in vitro assay of phagocytosis of neuroblastoma cells by iPSC-macrophages. Quantitative microscopy readouts are described for both live-cell time-lapse imaging and fixed-cell high-content imaging.

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In Vitro Quantitative Imaging Assay Phagocytosis Dead Neuroblastoma Cells IPSC-Macrophages Cell Treatments Genotypes Microglial Phagocytosis Neurodegenerative Disease High Content Imaging Screens Biological Safety Cabinet Dissociate SH-SY5Ys Cell Dissociation Buffer HBSS Phenol Red-free HEPES Buffered Media Fixation Formaldehyde Centrifuge Cell Pellet
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