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Journal / Neuroscience / In vitro Quantitative Imaging Assay für…
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Neuroscience

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JoVE Journal Neuroscience
In vitro Quantitative Imaging Assay für Phagozytose toter Neuroblastomzellen durch iPSC-Makrophagen
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In vitro Quantitative Imaging Assay für Phagozytose toter Neuroblastomzellen durch iPSC-Makrophagen

Article DOI: 10.3791/62217-v • 09:48 min • February 14th, 2021
February 14th, 2021 •
1,2,3, 2, 1, 2, 1
1James Martin Stem Cell Facility, Sir William Dunn School of Pathology, University of Oxford, 2Alzheimer’s Research UK Oxford Drug Discovery Institute, Nuffield Department of Medicine Research Building, University of Oxford, 3UK Dementia Research Institute, Cardiff University

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Neurodegenerative Erkrankungen sind mit dysregulierten Mikrogliafunktionen verbunden. Dieser Artikel beschreibt einen In-vitro-Assay der Phagozytose von Neuroblastomzellen durch iPSC-Makrophagen. Quantitative Mikroskopie-Auslesungen werden sowohl für die Zeitraffer-Bildgebung mit lebenden Zellen als auch für die High-Content-Bildgebung mit festen Zellen beschrieben.

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Neuroscience Phagozytose Efferozytose iPSC-Makrophage Mikroglia Neuron SH-SY5Y High-Content Lebendzelle Zeitraffer Neurodegeneration apoptotisch
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