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Journal / Medicine / マウス洞房・房室結節からの常在型心臓マクロファージの単離と培養…
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マウス洞房・房室結節からの常在型心臓マクロファージの単離と培養
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マウス洞房・房室結節からの常在型心臓マクロファージの単離と培養

Article DOI: 10.3791/62236-v • 08:43 min • May 7th, 2021
May 7th, 2021 •
1,2,3, 1, 1,3, 1, 1,2,3, 1,2,3, 1,2,3
1University Hospital Munich, Department of Medicine I, Ludwig-Maximilian-Unversity Munich (LMU), 2Insitute of Surgical Research at the Walter Brendel Center of Experimental Medicine, University Hospital Munich, Ludwig-Maximilians-University (LMU), 3German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA)

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ここで紹介するプロトコルは、マウス心臓の洞房結節(SAN)および房室結節(AVN)領域から心臓常在マクロファージを単離するための段階的なアプローチを提供します。

Tags

医学、第171号、心臓伝導系、洞房結節、房室結節、常在型心臓マクロファージ、フローサイトメトリー、磁気活性化細胞選別、MACS、蛍光活性化細胞選別、マイクロダイセクション、初代細胞培養
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