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Vurdering af proteininteraktioner i levende celler med FRET-sensibiliseret emission
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Vurdering af proteininteraktioner i levende celler med FRET-sensibiliseret emission

Article DOI: 10.3791/62241-v • 09:15 min • April 22nd, 2021
April 22nd, 2021 •
1, 2, 2, 2, 1, 2
1Department of Biophysics and Cell Biology, Faculty of Medicine, University of Debrecen, 2Gynecologic Oncology Division, Stanford University School of Medicine

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Förster Resonance Energy Transfer (FRET) mellem to fluoroformolekyler kan bruges til at studere proteininteraktioner i den levende celle. Her tilvejebringes en protokol for, hvordan man måler FRET i levende celler ved at detektere sensibiliseret emission af acceptoren og slukning af donormolekylet ved hjælp af konfokal laserscanningsmikroskopi.

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Biologi udgave 170 levende cellebilleddannelse FRET kvantitativ fluorescensmikroskopi fluorofor proteininteraktion sensibiliseret emission
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