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Vurdering av proteininteraksjoner i levende celler med FRET-sensibilisert utslipp
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Vurdering av proteininteraksjoner i levende celler med FRET-sensibilisert utslipp

Article DOI: 10.3791/62241-v • 09:15 min • April 22nd, 2021
April 22nd, 2021 •
1, 2, 2, 2, 1, 2
1Department of Biophysics and Cell Biology, Faculty of Medicine, University of Debrecen, 2Gynecologic Oncology Division, Stanford University School of Medicine

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Förster Resonance Energy Transfer (FRET) mellom to fluoroformolekyler kan brukes til å studere proteininteraksjoner i den levende cellen. Her er det gitt en protokoll for hvordan man måler FRET i levende celler ved å oppdage sensibilisert utslipp av akseptoren og slukke donormolekylet ved hjelp av konfokal laserskanningsmikroskopi.

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Biologi utgave 170 levende celleavbildning FRET kvantitativ fluorescensmikroskopi fluorofor proteininteraksjon sensibilisert emisjon
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