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Journal / Biology / Entrega do Complexo cas9/sgRNA Ribonucleoproteína …
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Entrega do Complexo cas9/sgRNA Ribonucleoproteína em Células Imortalizadas e Primárias através de Partículas semelhantes a vírus ("Nanoblades")
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Entrega do Complexo cas9/sgRNA Ribonucleoproteína em Células Imortalizadas e Primárias através de Partículas semelhantes a vírus ("Nanoblades")

Article DOI: 10.3791/62245-v • 09:23 min • March 31st, 2021
March 31st, 2021 •
2, 1, 1, 1
1LBMC, Laboratoire de Biologie et Modélisation de la Cellule Univ Lyon, ENS de Lyon, Université Claude Bernard Lyon 1, CNRS, UMR 5239, INSERM, U1210, Lyon, 69007, France, 2CIRI, Centre International de Recherche en Infectiologie Univ Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon, F-69007, Lyon, France

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Desenvolvemos um protocolo simples e barato para carregar complexos de ribonucleoproteína Cas9/guia único (sgRNA) dentro de partículas semelhantes a vírus. Essas partículas, chamadas "Nanoblades", permitem a entrega eficiente do complexo Cas9/sgRNA em células imortalizadas e primárias, bem como in vivo.

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Biologia Edição 169 CRISPR Cas9 ribonucleoproteína RNP Vírus da Leucemia Murina Partículas semelhantes a vírus VLP vetor viral entrega de proteínas
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