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Journal / Biology / Cryo-Structured Illumination Microscopic Data…
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Biology

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Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells

Article DOI: 10.3791/62274-v • 11:55 min • May 28th, 2021
May 28th, 2021 •
*1, *1, 1, 1, 1, 1,2, 1, 1, 3, 3, 4, 1
1Harwell Science and Innovation Campus, Beamline B24, Diamond Light Source, 2Division of Virology, Department of Pathology, University of Cambridge, 3ALBA Synchrotron, Beamline 09 - MISTRAL, 4Micron Advanced Imaging Consortium, Department of Biochemistry, University of Oxford
* These authors contributed equally

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Summary
Automatic Translation

This protocol demonstrates how to image biological cryo-preserved samples using cryo-structured illumination microscopy. We demonstrate the methodology by imaging the cytoskeleton of U2OS cells.

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Cryo-Structured Illumination Microscopy Cryo Imaging Super Resolution Imaging Cellular Structures Correlative Imaging Workflow Conventional Fluorophores Low Light Doses CryoSIM Cellular Ultrastructure Dynamics Vaccine Production Quality Control Post-market Surveillance Antibody Engineering Nanoparticle Characterization Cryo-stage Safety Cockpit Window Controls Liquid Nitrogen
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