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Recopilación de datos microscópicos de iluminación crioestructurada de células preservadas criogénicamente
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Recopilación de datos microscópicos de iluminación crioestructurada de células preservadas criogénicamente

Article DOI: 10.3791/62274-v • 11:55 min • May 28th, 2021
May 28th, 2021 •
*1, *1, 1, 1, 1, 1,2, 1, 1, 3, 3, 4, 1
1Harwell Science and Innovation Campus, Beamline B24, Diamond Light Source, 2Division of Virology, Department of Pathology, University of Cambridge, 3ALBA Synchrotron, Beamline 09 - MISTRAL, 4Micron Advanced Imaging Consortium, Department of Biochemistry, University of Oxford
* These authors contributed equally

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Este protocolo demuestra cómo obtener imágenes de muestras criopreservas biológicas utilizando microscopía de iluminación crioestructurada. Demostramos la metodología mediante la obtención de imágenes del citoesqueleto de las células U2OS.

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Biología Número 171 Imágenes criogénicas microscopía de fluorescencia imágenes 3D microscopía de superresión crioSIM microscopía de iluminación estructurada imágenes correlativas
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