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Journal / Biology / Direct Agroinoculation of Maize Seedlings by…
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Biology

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JoVE Journal Biology
Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones
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Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones

Article DOI: 10.3791/62277-v • 05:56 min • February 27th, 2021
February 27th, 2021 •
*1, *1, 1, 1
1Department of Plant Pathology and Microbiology, Iowa State University
* These authors contributed equally

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Summary
Automatic Translation

An Agrobacterium-based injection (agroinjection) protocol is presented for the inoculation of foxtail mosaic virus and sugarcane mosaic virusclones into maize seedlings. Inoculation in this manner leads to viral infection, virus-induced gene silencing of marker genes, and viral overexpression of GFP.

Tags

Direct Agroinoculation Maize Seedlings Injection Recombinant Foxtail Mosaic Virus Sugarcane Mosaic Virus Infectious Clones Viral Vectors Lab Settings Specialized Equipment Alternative Host Time-saving Resource-saving Additional Viral Vectors Maize Lines Monocot Species Planting Maize Seeds Peat-based Growing Medium Growth Chamber Greenhouse Water Plants Fertilize Agrobacterium For Injection LB Media Antibiotics Incubate At 28 Degrees Celsius Shaking At 225 RPM Pellet Bacteria Wash Pellet With Deionized Water
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