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Staining and High-Resolution Imaging of Three-Dimensional Organoid and Spheroid Models
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Staining and High-Resolution Imaging of Three-Dimensional Organoid and Spheroid Models

Article DOI: 10.3791/62280-v • 07:35 min • March 27th, 2021
March 27th, 2021 •
*1, *1, 2, 2, 2, 2, 3, 4, 1
1Childhood Cancer & Cell Death (C3), Université de Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Centre de recherche en cancérologie de Lyon (CRCL), Lyon 69373, 2Plateforme Anatomopathologie Recherche, Université de Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Centre de recherche en cancérologie de Lyon (CRCL), 3Plateforme d’Imagerie cellulaire, Université de Lyon, Université Claude Bernard Lyon 1, INSERM 1052, CNRS 5286, Centre Léon Bérard, Centre de recherche en cancérologie de Lyon (CRCL), 4PerkinElmer S.A.S.
* These authors contributed equally

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Summary
Automatic Translation

Here, we provide detailed, robust, and complementary protocols to perform staining and subcellular resolution imaging of fixed three-dimensional cell culture models ranging from 100 µm to several millimeters, thus enabling the visualization of their morphology, cell-type composition, and interactions.

Tags

Staining High-resolution Imaging Three-dimensional Organoid Models Spheroid Models Cellular Resolution Subcellular Resolution Fixed 3D In Vitro Models Staining Protocol Subcellular Resolution Imaging Embedding Techniques Visualized Instructions Whole Mount Staining Pipette Tip BSA PBS Permeabilization Blocking Solution
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