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Isolation of Primary Rat Hepatocytes with Multiparameter Perfusion Control
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Isolation of Primary Rat Hepatocytes with Multiparameter Perfusion Control

Article DOI: 10.3791/62289-v • 07:35 min • April 5th, 2021
April 5th, 2021 •
*1, *2, 3, 4, 5, 1, 3, 1,3,4,5,6
1Department of Physiology & The Institute for Digital Medicine (WisDM), National University of Singapore, 2College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, 3Mechanobiology Institute, National University of Singapore, 4Institute of Bioengineering and Nanotechnology, A*STAR, 5NUS Graduate School for Integrative Sciences and Engineering, 6CAMP, Singapore-MIT Alliance for Research and Technology
* These authors contributed equally

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Summary
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This protocol details the use of a special intravenous catheter, standardized sterile disposable tubing, temperature control complemented by real-time monitoring, and an alarm system for two-step collagenase perfusion procedure to improve the consistency in the viability, yield, and functionality of isolated primary rat hepatocytes.

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Isolation Primary Rat Hepatocytes Multiparameter Perfusion Control Liver Resection Digestion Approach Collagenase Recirculation Perfusion System Cell Isolation Gravity Yield Hepatocytes Specific Function Rat Abdominal Muscles Bile Duct Silk Surgical Suture Ligature Portal Vein Liver Lobes Gastric Vein Hole Tissue Cannulation Pressure Buildup Pump Speed Flow Rate Calcium Free Buffer Intravenous Catheter
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