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Isolering af primære rotte hepatocytter med multiparameter perfusionskontrol
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Isolering af primære rotte hepatocytter med multiparameter perfusionskontrol

Article DOI: 10.3791/62289-v • 07:35 min • April 5th, 2021
April 5th, 2021 •
*1, *2, 3, 4, 5, 1, 3, 1,3,4,5,6
1Department of Physiology & The Institute for Digital Medicine (WisDM), National University of Singapore, 2College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, 3Mechanobiology Institute, National University of Singapore, 4Institute of Bioengineering and Nanotechnology, A*STAR, 5NUS Graduate School for Integrative Sciences and Engineering, 6CAMP, Singapore-MIT Alliance for Research and Technology
* These authors contributed equally

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Denne protokol beskriver brugen af et specielt intravenøst kateter, standardiseret steril engangsslange, temperaturregulering suppleret med realtidsovervågning og et alarmsystem til to-trins kollagenaseperfusionsprocedure for at forbedre konsistensen i levedygtigheden, udbyttet og funktionaliteten af isolerede primære rotte hepatocytter.

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Biologi udgave 170 celleisolering hepatocyt totrins perfusion kanylering integreret enhed
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