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Aislamiento de hepatocitos primarios de rata con control de perfusión multiparamétrico
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Aislamiento de hepatocitos primarios de rata con control de perfusión multiparamétrico

Article DOI: 10.3791/62289-v • 07:35 min • April 5th, 2021
April 5th, 2021 •
*1, *2, 3, 4, 5, 1, 3, 1,3,4,5,6
1Department of Physiology & The Institute for Digital Medicine (WisDM), National University of Singapore, 2College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, 3Mechanobiology Institute, National University of Singapore, 4Institute of Bioengineering and Nanotechnology, A*STAR, 5NUS Graduate School for Integrative Sciences and Engineering, 6CAMP, Singapore-MIT Alliance for Research and Technology
* These authors contributed equally

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Este protocolo detalla el uso de un catéter intravenoso especial, tubos desechables estériles estandarizados, control de temperatura complementado con monitoreo en tiempo real y un sistema de alarma para el procedimiento de perfusión de colagenasa de dos pasos para mejorar la consistencia en la viabilidad, el rendimiento y la funcionalidad de los hepatocitos primarios aislados de ratas.

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Biología Número 170 aislamiento celular hepatocitos dos pasos perfusión canulación dispositivo integrado
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