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Confocal Laser Scanning Microscopy of Calcium Dynamics in Acute Mouse Pancreatic Tissue Slices
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Confocal Laser Scanning Microscopy of Calcium Dynamics in Acute Mouse Pancreatic Tissue Slices

Article DOI: 10.3791/62293-v • 10:49 min • April 13th, 2021
April 13th, 2021 •
1, 1,2, 1, 1, 1,3, 1
1Institute of Physiology, Faculty of Medicine, University of Maribor, 2Faculty of Natural Sciences and Mathematics, University of Maribor, 3Center for Physiology and Pharmacology, Medical University of Vienna

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Summary
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We present the preparation of acute pancreatic tissue slices and their use in confocal laser scanning microscopy to study calcium dynamics simultaneously in a large number of live cells, over long time periods, and with high spatiotemporal resolution.

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Confocal Laser Scanning Microscopy Calcium Dynamics Acute Mouse Pancreatic Tissue Slices Live Cell Calcium Imaging Intercellular Waves Multicellular Functional Connectivity Tissue Architecture Enzymatic Stress Mechanical Stress Pancreas-related Diseases Diabetes Brain Tissues Pituitary Tissues Adrenal Tissues Intercellular Contact Paracrine Interactions Agarose Injection
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