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Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification
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Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Article DOI: 10.3791/62295-v • 07:54 min • March 31st, 2021
March 31st, 2021 •
1,2,3, 1, 2,3,4, 1, 5, 1,2,3, 1,2,3, 1,2,3, 1, 1, 1
1Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, 2International College, University of Chinese Academy of Sciences, 3Sino-Africa Joint Research Center, 4CAS Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, 5Center for Infectious and Parasitic Diseases Control Research, Kenya Medical Research Institute

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Summary
Automatic Translation

This work summarizes steps on developing different assays for SARS-CoV-2 detection using a two color ddPCR system. The steps are elaborate and notes have been included on how to improve the assays and experiment performance. These assays may be used for multiple SARS-CoV-2 RT-ddPCR applications.

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Two-Step Reverse Transcription Droplet Digital PCR SARS-CoV-2 Detection SARS-CoV-2 Quantification Multiplex DdPCR Assays Two-color DdPCR System In-house Kits RT-qPCR RT-ddPCR CDNA Synthesis Reverse Transcription Master Mix RNA Sample Thermal Cycler Droplet Digital PCR Plate
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