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JoVE Journal Biology
To-trins omvendt transkriptionsdråbe digitale PCR-protokoller til SARS-CoV-2-detektion og kvantificering
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To-trins omvendt transkriptionsdråbe digitale PCR-protokoller til SARS-CoV-2-detektion og kvantificering

Article DOI: 10.3791/62295-v • 07:54 min • March 31st, 2021
March 31st, 2021 •
1,2,3, 1, 2,3,4, 1, 5, 1,2,3, 1,2,3, 1,2,3, 1, 1, 1
1Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, 2International College, University of Chinese Academy of Sciences, 3Sino-Africa Joint Research Center, 4CAS Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, 5Center for Infectious and Parasitic Diseases Control Research, Kenya Medical Research Institute

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Dette arbejde opsummerer trin til udvikling af forskellige assays til SARS-CoV-2-detektion ved hjælp af et tofarvet ddPCR-system. Trinene er detaljerede, og der er inkluderet noter om, hvordan man forbedrer assays og eksperimentets ydeevne. Disse assays kan bruges til flere SARS-CoV-2 RT-ddPCR-applikationer.

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Denne måned i JoVE udgave 169 simplex duplex triplex sondeblanding quadruplex assays SARS-CoV-2 RT-ddPCR detektion multiplex
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