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JoVE Journal
Immunology and Infection
Author Produced

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基因敲击由CRISPR/Cas9和细胞排序在巨噬细胞和T细胞线
 
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基因敲击由CRISPR/Cas9和细胞排序在巨噬细胞和T细胞线

Article DOI: 10.3791/62328-v
November 13th, 2021

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Summary November 13th, 2021

Please note that all translations are automatically generated.

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该协议使用荧光记者和细胞分拣来简化巨噬细胞和T细胞系的敲击实验。两个质粒用于这些简化的敲击实验,即CRISPR/Cas9-和DsRed2表达质粒和同源重组供体质粒表达EBFP2,这是永久整合在 Rosa26 点在免疫细胞。

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