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Journal / Immunology and Infection / MACROPHAGE ve T Hücre Hatlarında CRISPR/Cas9 ve…
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Immunology and Infection
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JoVE Journal Immunology and Infection
MACROPHAGE ve T Hücre Hatlarında CRISPR/Cas9 ve Hücre Sıralama tarafından Gen Knock-in
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MACROPHAGE ve T Hücre Hatlarında CRISPR/Cas9 ve Hücre Sıralama tarafından Gen Knock-in

Article DOI: 10.3791/62328-v • 11:32 min • November 13th, 2021
November 13th, 2021 •
1, 1, 1, 1, 1, 1, 1, 1, 2, 1
1The Laboratory of Genetic Regulators in the Immune System, School of Laboratory Medicine, Xinxiang Medical University, 2Centre d’Immunologie de Marseille-Luminy, Aix Marseille Université

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Bu protokol, makrofaj ve T hücre satırlarındaki darbe denemelerini basitleştirmek için floresan muhabirleri ve hücre sıralamasını kullanır. Bu basitleştirilmiş knock-in deneyleri için iki plazmid kullanılır, yani CRISPR / Cas9- ve DsRed2 ifade eden plazmid ve bağışıklık hücrelerindeki Rosa26 lokusunda kalıcı olarak entegre edilen EBFP2'yi ifade eden homolog bir rekombinasyon donör plazmid.

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İmmünoloji ve Enfeksiyon Sayı 177 knock-in CRISPR/Cas9 Rosa26,makrofaj T hücresi hACE2
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