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Journal / Bioengineering / DNA-spændingssonder til at kortlægge…
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
DNA-spændingssonder til at kortlægge immuncellernes forbigående piconewtonreceptorkræfter
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Journal (Bioengineering)
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Journal (Bioengineering)
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DNA-spændingssonder til at kortlægge immuncellernes forbigående piconewtonreceptorkræfter

Article DOI: 10.3791/62348-v • 06:53 min • March 20th, 2021
March 20th, 2021 •
1, 2, 1, 1, 2, 1,2
1Department of Chemistry, Emory University, 2Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University

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Dette papir beskriver en detaljeret protokol til brug af DNA-baserede spændingsprober til at afbilde receptorkræfterne anvendt af immunceller. Denne tilgang kan kortlægge receptorkræfter >4,7 pN i realtid og kan integrere kræfter over tid.

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Denne måned i JoVE udgave 169 DNA-spændingsprober mekanobiologi receptorkraft piconewtonkræfter immunceller kortlægning
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