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冷冻电子断层扫描 的糖核 酸的准备和冷冻-FIB微加工
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Article
DOI:
10.3791/62351-v
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09:06 min
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November 20th, 2021
冷冻电子断层扫描 的糖核 酸的准备和冷冻-FIB微加工
November 20th, 2021
•
Summary
Please note that all translations are automatically generated.
Click here for the English version.
我们提出了一个协议,通过以低温为中心的离子束微加工,为大分子 进行高 分辨率结构研究,现场用低温电子断层扫描制备小型冷冻生物标本。提交的协议为制备高质量的拉梅莱提供了指导方针,具有高可重复性,用于在萨查罗米切斯宫内进行大分子的结构特征。