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JoVE Journal Biochemistry
Forberedelse og Cryo-FIB mikromaskinering av Saccharomyces cerevisiae for Cryo-Electron Tomography
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Forberedelse og Cryo-FIB mikromaskinering av Saccharomyces cerevisiae for Cryo-Electron Tomography

Article DOI: 10.3791/62351-v • 09:06 min • November 20th, 2021
November 20th, 2021 •
1, 1, 1, 1
1Central European Institute of Technology, Masaryk University

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Vi presenterer en protokoll for lamellforberedelse av dypfryste biologiske prøver ved kryofokusert ionstrålemikromaskinering for høyoppløselige strukturelle studier av makromolekyler in situ med kryo-elektrontomografi. Den presenterte protokollen gir retningslinjer for fremstilling av høykvalitets lameller med høy reproduserbarhet for strukturell karakterisering av makromolekyler inne i Saccharomyces cerevisiae.

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