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Strategier for optimering af kryogene Electron Tomography Data Acquisition
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Strategier for optimering af kryogene Electron Tomography Data Acquisition

Article DOI: 10.3791/62383-v • 08:16 min • March 19th, 2021
March 19th, 2021 •
1, 1, 2, 1,3
1Structural and Computational Biology Unit, European Molecular Biology Laboratory, 2Electron Microscopy Core Facility, European Molecular Biology Laboratory, 3Imaging Centre, European Molecular Biology Laboratory

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Den stigende efterspørgsel efter storstilet dataindsamling i kryogene elektrontomografi kræver høj-throughput billedopsamling rutiner. Beskrevet her er en protokol, der gennemfører den seneste udvikling af avancerede erhvervelsesstrategier, der sigter mod at maksimere tidseffektiviteten og gennemløbet af tomografisk dataindsamling.

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Biologi Problem 169 kryogen elektrontomografi automatisering høj gennemløb PyEM SerialEM
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