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In vitro Analisi della funzione E3 Ubiquitina ligasi
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In vitro Analisi della funzione E3 Ubiquitina ligasi

Article DOI: 10.3791/62393-v • 06:06 min • May 14th, 2021
May 14th, 2021 •
1, 1,2, 1, 1,2
1Institute for Genetics and Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases (CECAD), University of Cologne, 2Center for Molecular Medicine Cologne, University of Cologne

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Il presente studio fornisce protocolli dettagliati di test di ubiquitilazione in vitro per l'analisi dell'attività catalitica dell'ubiquitina ligasi E3. Le proteine ricombinanti sono state espresse utilizzando sistemi procariotici come la coltura di Escherichia coli.

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Biologia Numero 171
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