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In vitro Analyse de la fonction E3 Ubiquitin Ligase
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In vitro Analyse de la fonction E3 Ubiquitin Ligase

Article DOI: 10.3791/62393-v • 06:06 min • May 14th, 2021
May 14th, 2021 •
1, 1,2, 1, 1,2
1Institute for Genetics and Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases (CECAD), University of Cologne, 2Center for Molecular Medicine Cologne, University of Cologne

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La présente étude fournit des protocoles détaillés de dosage d’ubiquitylation in vitro pour l’analyse de l’activité catalytique de l’ubiquitine ligase E3. Les protéines recombinantes ont été exprimées à l’aide de systèmes procaryotes tels que la culture d’Escherichia coli.

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Biologie numéro 171
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