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Journal / Biology / In vitro Analyse der E3-Ubiquitin-Ligase-Funktion
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In vitro Analyse der E3-Ubiquitin-Ligase-Funktion
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In vitro Analyse der E3-Ubiquitin-Ligase-Funktion

Article DOI: 10.3791/62393-v • 06:06 min • May 14th, 2021
May 14th, 2021 •
1, 1,2, 1, 1,2
1Institute for Genetics and Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases (CECAD), University of Cologne, 2Center for Molecular Medicine Cologne, University of Cologne

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Die vorliegende Studie liefert detaillierte In-vitro-Ubiquitylierungs-Assay-Protokolle für die Analyse der katalytischen Aktivität der E3-Ubiquitin-Ligase. Rekombinante Proteine wurden mit prokaryotischen Systemen wie der Escherichia coli-Kultur exprimiert.

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Biologie Heft 171
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