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In vitro Análisis de la función E3 Ubiquitina Ligasa
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In vitro Análisis de la función E3 Ubiquitina Ligasa

Article DOI: 10.3791/62393-v • 06:06 min • May 14th, 2021
May 14th, 2021 •
1, 1,2, 1, 1,2
1Institute for Genetics and Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases (CECAD), University of Cologne, 2Center for Molecular Medicine Cologne, University of Cologne

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El presente estudio proporciona protocolos detallados de ensayo de ubiquitilación in vitro para el análisis de la actividad catalítica de la ubiquitina ligasa E3. Las proteínas recombinantes se expresaron utilizando sistemas procariotas como el cultivo de Escherichia coli.

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Biología Número 171
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