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JoVE Journal
Biology

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In Vitro E3 Ubiquitin Ligase fonksiyonunun analizi
 
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In Vitro E3 Ubiquitin Ligase fonksiyonunun analizi

Article DOI: 10.3791/62393-v
May 14th, 2021

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Summary May 14th, 2021

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Bu çalışma, E3 ubiquitin ligase katalitik aktivitesinin analizi için ayrıntılı in vitro ubiquitylation tahlil protokolleri sunmaktadır. Rekombinant proteinler Escherichia coli kültürü gibi prokaryotik sistemler kullanılarak ifade edildi.

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