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Biochemistry

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JoVE Journal Biochemistry
15 N CPMG Relaxation Dispersão para a Investigação da Dinâmica Conformacional de Proteínas na escala de tempo μs-ms
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15 N CPMG Relaxation Dispersão para a Investigação da Dinâmica Conformacional de Proteínas na escala de tempo μs-ms

Article DOI: 10.3791/62395-v • 08:09 min • April 19th, 2021
April 19th, 2021 •
1, 1, 1,2
1Department of Chemistry, Iowa State University, 2Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University

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Aqui, é fornecida uma descrição detalhada do protocolo implementado no laboratório para aquisição e análise de perfis de dispersão de relaxamento de 15N por espectroscopia de solução NMR.

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Bioquímica Edição 170 Relaxamento de RMN dinâmica de proteínas dispersão de relaxamento Carr-Purcell Meiboom-Gill perditeration TROSY
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