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Kvantitative metoder for å studere protein Arginin Methyltransferase 1-9 Aktivitet i celler
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Kvantitative metoder for å studere protein Arginin Methyltransferase 1-9 Aktivitet i celler

Article DOI: 10.3791/62418-v • 08:11 min • August 7th, 2021
August 7th, 2021 •
1, 1, 1,2
1Structural Genomics Consortium, University of Toronto, 2Department of Pharmacology and Toxicology, University of Toronto

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Disse protokollene gir metodikken som brukes til å vurdere den enzymatiske aktiviteten til individuelle medlemmer av protein arginin metyltransferase (PRMT) familien i celler. Detaljerte retningslinjer for vurdering av PRMT-aktivitet ved hjelp av endogene og eksogene biomarkører, metyl-arginin som gjenkjenner antistoffer, og inhibitorverktøyforbindelser er beskrevet.

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