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Isolering af mus interstitielle ventilceller til undersøgelse af forkalkning af aortaklappen In Vitro
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Isolering af mus interstitielle ventilceller til undersøgelse af forkalkning af aortaklappen In Vitro

Article DOI: 10.3791/62419-v • 05:47 min • May 10th, 2021
May 10th, 2021 •
1, 1,2,3
1Cardiovascular Research Center, The Icahn School of Medicine at Mount Sinai, 2Diabetes, Obesity and Metabolism Institute, Department of Medicine, The Icahn School of Medicine at Mount Sinai, 3Graduate School of Biomedical Sciences, The Icahn School of Medicine at Mount Sinai

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I denne artikel beskrives isoleringen af musaortaklappeceller ved hjælp af en totrins kollagenprocedure. Isolerede museventilceller er vigtige for at udføre forskellige assays, såsom denne in vitro-forkalkningsanalyse, og for at undersøge de molekylære veje, der fører til aortaklappens mineralisering.

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