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Investigating the Phagocytosis of Leishmania using Confocal Microscopy
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Immunology and Infection
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JoVE Journal Immunology and Infection
Investigating the Phagocytosis of Leishmania using Confocal Microscopy

Investigating the Phagocytosis of Leishmania using Confocal Microscopy

DOI:
10.3791/62459-v

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08:41 min

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July 29, 2021

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, , , , , ,
1Laboratory of Host-Parasite Interaction and Epidemiology,Gonçalo Moniz Institute, 2National Institute of Science and Technology of Tropical Diseases – National Council for Scientific Research and Development (CNPq)

Chapters

  • 00:05Introduction
  • 01:20Leishmania Phagocytosis Assessment by Macrophages
  • 02:50Evaluation of Leishmania‐Induced Vacuole Maturation
  • 04:55Confocal Microscopy Acquisition and Quantification
  • 06:08Results: Assessment of Leishmania spp. Phagocytosis
  • 07:43Conclusion

Summary

Automatic Translation

Automatic Translation

The mechanism associated with phagocytosis in Leishmania infection remains poorly understood. Here, we describe methods to evaluate the early events occurring during Leishmania interaction with the host cells.

Tags

Keywords: PhagocytosisLeishmaniaConfocal MicroscopyTHP-1 CellsMacrophagesPromastigotesPV BiogenesisTransfection

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