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Biochemistry

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JoVE Journal Biochemistry
Nano-differentialscanning fluormetri til screening i fragmentbaseret lead discovery
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Nano-differentialscanning fluormetri til screening i fragmentbaseret lead discovery

Article DOI: 10.3791/62469-v • 06:26 min • May 16th, 2021
May 16th, 2021 •
1, 1, 1, 2, 2, 2, 2, 1, 1
1Oncode Institute and Division of Biochemistry, the Netherlands Cancer Institute, 2Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe-University

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Overvågning af ændringer i smeltetemperaturen af et målprotein (dvs.termisk skiftanalyse, TSA) er en effektiv metode til screening af fragmentbiblioteker af et par hundrede forbindelser. Vi præsenterer en TSA-protokol, der implementerer robotassisteret nanodifferenti differentialscanning af fluormetri (nano-DSF) til overvågning af iboende tryptofanlyscens og let rygspredning til fragmentscreening.

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Biokemi Thermal shift assay fragment screening drug design SARS-CoV-2 Nsp5 Hec1 Mps1
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