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JoVE Journal
Biochemistry

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Nano-differensialskanning fluorimetri for screening i fragmentbasert blyoppdagelse
 
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Nano-differensialskanning fluorimetri for screening i fragmentbasert blyoppdagelse

Article DOI: 10.3791/62469-v
May 16th, 2021

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Summary May 16th, 2021

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Click here for the English version.

Overvåking av endringer i smeltetemperaturen til et målprotein (dvs.termisk skiftanalyse, TSA) er en effektiv metode for screening av fragmentbiblioteker av noen få hundre forbindelser. Vi presenterer en TSA-protokoll som implementerer robotisert nano-differensialskanning Fluorimetry (nano-DSF) for overvåking av iboende tryptofan fluorescens og lys back-scattering for fragmentscreening.

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