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JoVE Journal Cancer Research
Modeling the Effects of Hemodynamic Stress on Circulating Tumor Cells using a Syringe and Needle
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Modeling the Effects of Hemodynamic Stress on Circulating Tumor Cells using a Syringe and Needle

Article DOI: 10.3791/62478-v • 05:49 min • April 27th, 2021
April 27th, 2021 •
1,2, 3, 1, 1, 1,2,4,5,6
1Department of Molecular Physiology and Biophysics, Carver College of Medicine, University of Iowa, 2Holden Comprehensive Cancer Center, University of Iowa, 3MD program, Carver College of Medicine, University of Iowa, 4Department of Pathology, Carver College of Medicine, University of Iowa, 5Department of Urology, Carver College of Medicine, University of Iowa, 6Department of Radiation Oncology, Carver College of Medicine, University of Iowa

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Summary
Automatic Translation

Here we demonstrate a method to apply fluid shear stress to cancer cells in suspension to model the effects of hemodynamic stress on circulating tumor cells.

Tags

Hemodynamic Stress Circulating Tumor Cells Syringe Needle Fluid Shear Stress Metastatic Cancer Cells Circulatory System Safety Measures PC3 Cells Trypsinization Cell Suspension Cell Concentration Total Cell Number Centrifuge Supernatant Serum-free Tissue Culture Medium
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