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Immunology and Infection

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Detecção de anticorpos neutralizantes SARS-CoV-2 usando imagens fluorescentes de alto rendimento da infecção por pseudovírus

Article DOI: 10.3791/62486-v • 10:25 min • June 5th, 2021
June 5th, 2021 •
1,2, 1,2, 1,2, 1, 1,2, 1,2, 1,2
1Ottawa Hospital Research Institute, 2Department of Biochemistry, Microbiology and Immunology, University of Ottawa

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O protocolo descrito aqui descreve um método rápido e eficaz para medir anticorpos neutralizantes contra a proteína de pico SARS-CoV-2, avaliando a capacidade de amostras de soro convalescente para inibir a infecção por um vírus de estomatite vesicular rotulado por proteína verde aumentada com pseudotipto de glicoproteína.

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Imunologia e Infecção Problema 172 SARS-CoV-2 COVID-19 Pseudovírus Anticorpo Neutralizante vírus da estomatite vesicular glicoproteína de pico
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