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JoVE Journal Bioengineering
Análise constante da mola molecular por espectroscopia da sonda de força biomembrana
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Análise constante da mola molecular por espectroscopia da sonda de força biomembrana

Article DOI: 10.3791/62490-v • 08:10 min • November 20th, 2021
November 20th, 2021 •
1, 1,2,3, 1, 1,4, 1, 5, 5, 1,2, 1,2,3
1School of Biomedical Engineering, Faculty of Engineering, The University of Sydney, 2Charles Perkins Centre, The University of Sydney, 3Heart Research Institute, 4Department of Chemistry, The Hong Kong University of Science and Technology, 5School of Aerospace, Mechanical and Mechatronic Engineering, Faculty of Engineering, The University of Sydney

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Uma sonda de força biomembrana (BFP) é uma técnica in situ dynamic force spectroscopy (DFS). O BFP pode ser usado para medir a constante de mola das interações moleculares em células vivas. Este protocolo apresenta análises constantes de mola para ligações moleculares detectadas pela BFP.

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Bioengenharia Edição 177 Constante da mola molecular sonda de força biomembana espectroscopia de força dinâmica ensaio de estiramento integrin
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