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JoVE Journal Biology
In Situ Udforskning af Murine Megakaryopoiesis ved hjælp af Transmission ElektronMikroskopi
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In Situ Udforskning af Murine Megakaryopoiesis ved hjælp af Transmission ElektronMikroskopi

Article DOI: 10.3791/62494-v • 08:15 min • September 8th, 2021
September 8th, 2021 •
1, 1, 1, 1
1Université de Strasbourg, INSERM, EFS Grand Est, BPPS UMR-S 1255, FMTS

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Her præsenterer vi en protokol til at analysere ultrastruktur af megakaryocytterne in situ ved hjælp af transmissionselektronmikroskopi (TEM). Murin knoglemarv indsamles, fast, indlejret i epoxyharpiks og skæres i ultratynde sektioner. Efter kontrastfarvning observeres knoglemarven under et TEM-mikroskop ved 120 kV.

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Biologi Udgave 175 megakaryocyt trombopoiesis knoglemarv transmissionselektronmikroskopi in situ
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