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In situ Exploratie van Murine Megakaryopoiesis met behulp van Transmissie ElektronenMicroscopie

Article DOI: 10.3791/62494-v • 08:15 min • September 8th, 2021
September 8th, 2021 •
1, 1, 1, 1
1Université de Strasbourg, INSERM, EFS Grand Est, BPPS UMR-S 1255, FMTS

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Hier presenteren we een protocol om de ultrastructuur van de megakaryocyten in situ te analyseren met behulp van transmissie-elektronenmicroscopie (TEM). Murine beenmerg wordt verzameld, gefixeerd, ingebed in epoxyhars en gesneden in ultradunne secties. Na contrastkleuring wordt het beenmerg waargenomen onder een TEM-microscoop bij 120 kV.

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Biologie Nummer 175 megakaryocyt trombopoiese beenmerg transmissie elektronenmicroscopie in situ
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